Pcna^tm1Jcbs

An A to G transition was targeted at exon 4 of the locus to mutate residue 164 from a lysine to an arginine (K164R). For targeting, a Frt-flanked PGK selection cassette was inserted into intron 1 with one loxP site upstream of the selection cassette. Due to the presence of several psuedogenes that make targeting difficult, homologous recombination of ES cells was verified by multiplex ligation-dependent probe amplification using primers with intronic sequence. (J:126595)