Tg(Lck-ERT2/Tal1)AJrg

The expressed sequence is a chimeric cDNA encoding the tamoxifen-responsive mutant human estrogen receptor ligand-binding domain ERT2 joined, via a 9-aa flexible linker peptide, to mouse T-cell acute lymphocytic leukemia 1 (TAL1/SCL) truncated by 108 bp at its N-terminus. The cDNA was inserted into the p1017 expression vector between the mouse thymocyte-specific, proximal Lck promoter and a human growth hormone minigene beginning within exon 1 and containing splice donor/acceptor sequences and the polyadenylation signal. Thymic mRNA expression was confirmed by RT-PCR analysis. Immunohistochemical analysis of thymic sections with an anti-estrogen receptor antibody demonstrated nuclear translocation of most of the fusion protein following treatment of the mice with OHT, but its retention in the cytoplasm in the absence of OHT. (J:79835, J:81268, J:125689)