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包含IgH-6基因(μ重链)和反向Ighg1(μ重链γ1恒定区)的DNA片段被插入在常量重链基因区域(IGH)的下游，以限制可溶性异构体的表达。通过突变(Ighg1)或消除(Igh-6)相关的polyA位点，实现了这一点。周围设置了LoxP位点，随后插入了抗氨苄青霉素抗性基因。这个构建用于Igh-6tm1Fbb小鼠的干细胞靶向，其中Igh-6基因座已被LoxP位点替换。杂交小鼠与表达Cre重组酶的转基因小鼠杂交，去除了IGH整个区域和neo抗性基因，但保留了突变的Igh-6基因，且定向正确表达。这些小鼠的B细胞确证只分泌膜结合的μ抗体。(来源：J:125719)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
