Tes^{Gt(RRH061)Byg}

The gene trap vector was inserted between exons 3 and 4 disrupting the PET and LIM domains. The absence of a protein product was confirmed by western blot analysis on protein extracts from E10.5 embryos. These allele produces a fusion protein containing 133 amino acids fused to the beta-geo cassette. Since only 32 amino acids of the 104 amino acid PET domain are present in the fusion product this allele is expected to be a null allele. (J:125765)