F10^tm2Ccmt

Exon 8 contains the missense mutation encoding Factor X Friuli (Pro343Ser). This allele was generated by targeting the "socket" F10tm1Ccmt allele with a "plug" that introduced the Friuli mutation and simultaneously eliminated the neomycin resistance gene and reconstituted the Hprt gene, allowing selection of correctly targeted ES cells for HAT resistance and G418 sensitivity. Extrinsic and intrinsic protein activity is decreased to 5% of wild-type. (J:145092)