部分exon2和exon3被替换了EGFP-PGK-neo cassette。通过使用Mapkap1特异性的抗体K87检测,对鼠胚胎成纤维细胞提取物进行 Western blot 来确认没有蛋白质表达。(来源:J:125618)
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Part of exon 2 and exon 3 were replaced with an EGFP-PGK-neo cassette. The lack of protein expression was confirmed by a western blot of extracts from mouse embryonic fibroblast cells using a Mapkap1-specific antibody K87. (J:125618)