Matn3^tm1Mbri

The amino acid substitution of a valine for an aspartic acid at position 149 (p.Val194Asp) was inserted into exon 2 through nucleotide substitution (GTG to GAT). A floxed neo cassette used as a selectable marker was removed by cre-mediated recombination using the pIC-Cre vector transfected into ES cells. This mutation is equivalent to a human disease causing mutation ioin the A-domain. Correct insertion of the mutation was confirmed by sequencing. (J:125104)