2号和3号外显子通过Cre介导的重组被去除,同时删除了neo cassette,蛋白质表达缺失通过 Western blot 用两种抗体(528B和639B)进行了确认(来源:J:125344)
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Exons 2 and 3 were removed by cre-mediated recombination that also removed the neo cassette. The loss of protein expression was confirmed by western blot analysis using two antibodies (528B and 639B). (J:125344)