Tg(Th-SNCA*)1Mgsp

The construct consisted of the sequence expressing human alpha-synuclein 1-120 which is truncated, lacking the final 20 amino acids inserted downstream of the rat tyrosine hydroxylase promoter Th in an expression vector. Vector sequences were removed and the linearized construct was microinjected into fertilized eggs. These mice were crossed to C57BL/6JOlaHsd mice which lack alpha-synuclein as a result of a spontaneous deletion of the alpha-SNCA locus. Immunoblotting demostrated high levels of alpha-synuclein 1-120 in the nigrostriatal pathway and the olfactory bulb in the line 1 founder. (J:108310)