Tg(dlx5a-cre)1Mekk

A cre gene was placed under the control of the I56i and I56ii enhancer elements of zebrafish dlx5a and a 3.5kb genomic DNA fragment flanking the 5' region of the zebrafish dlx6a open reading frame and containing the 5'UTR. The dlx6a flanking region on its own cannot produce tissue specific expression but increases the activity of the enhancers to produce expression that recapitulate endogenous Dlx5 expression. Tissue specific expression was confirmed by test crosses to reporter mice strains, including Tg(ACTB-Bgeo/ALPP)1Lbe, Tg(ACTB-Bgeo/GFP)21Lbe and Gt(ROSA)26Sortm1Sor. (J:122066)