Exon 1被替换为一个无启动子的核定位信号标记的lacZ/PGK-neo插入片段。通过 Western blot 在肝脏匀浆中检测,确认没有蛋白质产品产生。(来源:J:124917)
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Exon 1 was replaced with a promoterless nuclear localization signal-tagged lacZ/PGK-neo cassette. The absence of protein product was confirmed by western blot analysis on liver lysates. (J:124917)