Spr^tm1Spo

A portion of exon 1, intron 1 and all of exon 2 were replaced with a PGK-neo cassette. The absence of full length transcript was confirmed by northern blot. Exon 2 encodes a short chain dehydrogenase/reductase domain that is essential for Spr function and its deletion and resulting frameshift is expected to produce a null allele. (J:124817)