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Using a targeting vector constructed with 129/Sv mouse DNA, a floxed neo cassette was inserted upstream of exon 5 and an additional lox P site was inserted downstream of exon 7. Cre-mediated recombination removed the neo cassette and exons 5 through 7 to create a truncated protein detectable by western blot analysis that lacks a complete PDZ dmain and leucine zipper. (J:124994)