设计了一个靶向载体,用于将新霉素抗性基因座插入到替换exon 4的位置。通过 Western blot 分析确认了蛋白缺失。(来源:J:124858)
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A targeting vector was designed to insert a neomycin resistance cassette into the locus to replace exon 4. Absence of protein was confirmed by Western blot analysis. (J:124858)