C8b⁰

A screen of sera from mice of 73 strains for deficiencies in complement-mediated lysis of antibody-primed rabbit or sheep erythrocytes identified two strains, of 8 derived from the wild hybrid species M. m. molossinus, lacking lytic activity. Their hemolytic activity was restored by addition of partially purified human complement factor 8 or of human serum depleted of C8alpha and C8gamma. The gene responsible in strain MSM/Ms was mapped to the region of the mouse genome sharing synteny with the chromosomal location of human C8B. Combined sera from the two deficient strains also lacked hemolytic activity. (J:11000)