Nlrp3^tm1Tsc

The gene was disrupted by the insertion of an EGFP cassette fused in frame with the ATG of exon 2. A selection cassette PGK-neo flanked by 2 loxP sites was also inserted in the intron 2. The neo cassette was deleted by backcrossing the mice with a Cre-expressing deleter strain. RT-PCR analysis of RNA isolated from mutant macrophages confirmed that the wild-type transcript is absent. (J:124226)