将En1的cDNA和825个碱基对的E. Coli lacZ片段插入在pWEXP2质粒的EcoRV位点。该质粒包含一个经过修改的Wnt1基因片段,其中Wnt1的起始密码子已被替换为含有NruI、EcoRV、SacII和BstBI限制酶切割位点的polylinker区域。(来源:J:16386, J:35491)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count