通过将人类KLF4基因的1-1780个碱基片段与MMTV启动子驱动的载体片段连接,构建了一个 constitutive expression construct。这个4.7kb的片段被注入到(C57BL/6 x SJL)F2的卵子中。(来源:J:96886)
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A constitutive expression construct was generate by ligating a fragment comprising bases 1-1780 of the human KLF4 gene to a vector fragment with the MMTV promoter. The 4.7 kb fragment was injected into (C57BL/6 x SJL)F2 ova. (J:96886)