Ambra1^{Gt(pGT1.8geo)1Fcec}

The gene trap insertion was located between the 100 and 113-bp coding exons and disrupted the protein at amino acid position 841, resulting in the deletion of 459 amino acids from the C-terminal end. A fusion transcript was detected and the endogenous protein was absent, as shown by Northern and Western blots, respectively. (J:122795)