通过Cre介导的重组,我们从tm1等位基因中去除了exon 2,从而产生了无功能的突变体。蛋白产物的缺失通过 Western blot 分析得到了确认。(来源:J:122459)
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Cre-mediated recombination was used to remove exon 2 from the tm1 allele to create a null allele. The absence of protein product was confirmed by western blot analysis. (J:122459)