设计了一种靶向载体,它将来自大鼠神经素1α的信号肽,随后是GFP的"翡翠"元件连接到exon1的氨基端,exon2两侧插入loxP位点,并在内含子2中插入一个由Flp标签的诺卡因抗性基因。实验后,通过western blot分析检测到了GFP融合蛋白的存在。(来源:J:122459)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count