Nkd1^tm1Kwha

A targeting vector was designed to insert a stop codon into exon 5 at amino acid 91, as well as replace exons 5-10 of the targeted gene with an internal ribosome entry site-b-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Northern blot analysis of lung tissue and Western blot analysis of embronic tissue in homozygotes confirm lack of normal mRNA expression, while X-gal staining closely mimics dynamic expression pattern of normal Nkd1 mRNA. (J:122353)