通过Cre介导的重组,去除了locus上的floxed neo,替换了为zing finger protein 423的全长cDNA,然后插入了IRES-3NLS-YFP-pA,取代了该区域的前五个外显子。(来源:J:122275)
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The floxed neo was removed from the locus via cre mediated recombination, leaving the full length cDNA for zing finger protein 423, followed by IRES-3NLS-YFP-pA in place of the first five exons of the locus. (J:122275)