在exon 2的上游插入了loxP位点和一个含氨苄青霉素抗性基因的弗里特-flanked序列,然后在exon 2下游1kb处插入了另一个loxP位点和EcoRI限制酶切点。通过Southern blotting和基因组PCR确认了目标插入的准确性。(来源:J:139846)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count