Tg(Camk2a-Crebbp*)1364Tabe

A transgenic construct was designed with a FLAG-epitope sequence tag at the amino terminus of a truncated mouse CREB-binding protein sequence (called CBPdelta1; lacking the coding sequence for amino acids 1084-2441), all flanked by an artificial intron and splice sites at the 5' end and by a polyadenylation signal from SV40 at the 3' end. This FLAG-CBPD1 sequence was then placed downstream of 8.5 kb of the mouse CaMKIIalpha promoter. The resulting 13 kb "CaMKIIalpha-FLAG-CBPdelta1" transgene was microinjected into the pronuclei of B6SJLF1 zygotes. Expression of the truncated product is restricted to the post-natal hippocampus, striatum, amygdala, and cortex. (J:121812)