在第8个内含子中插入了lacZ基因陷阱,并移除了编码催化区域部分的9-14号外显子。 Western blot结果显示突变胚胎中没有蛋白质表达。(来源:J:121697)
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A gene trap containing lacZ was inserted into intron 8 and removed exons 9-14, which encode portions of the catalyitic domain. Western blot demonstrated absence of protein from mutant embryos. (J:121697)