Braf^tm1Mmcm

A targeting vector was designed to insert a loxP site and the human BRAF cDNA covering exons 15-18 into intron 14, along with a floxed neo cassette. A modified exon 15 was engineered into the vector to encode BrafV600E and a silent XbaI restriction site. RT-PCR confirmed that mutant transcript was expressed at the same level as wild-type, and that the V600E mutation was not detected. (J:121715)