Elovl4^tm1Wked

A targeting vector was designed to insert a floxed neo and DNA construct containing a pathogenic 5-bp deletion ((797-801_AACTT) and two point mutations in exon 6. This construct resulted in a truncated protein which contains the same aberrant 8-amino acid C-terminus encoded by the human pathogenic allele. The floxed neo was subsequently removed via cre mediated recombination. (J:121481)