在第3外显子中插入了一个新霉素抗性基因座,通过RT-PCR确认了neo基因的插入。通过 Western blot 分析,证实了没有蛋白质表达(来源:J:121262)
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A neomycin resistance cassette was inserted into exon 3 in frame. RT-PCR confirmed insertion of the neo and absence of protein was demonstrated by Western blot analysis. (J:121262)