设计了一个靶向载体,用于将3-5号外显子替换为一个含荧光素诺卡因抗性基因座的敲除元件。 Western blot结果显示,突变大脑、肌肉和睾丸中没有检测到蛋白质(来源:J:120741)
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A targeting vector was designed to replace exons 3-5 with a floxed neomycin resistance cassette. Western blot confirmed absence of protein in mutant brain, muscle and testis. (J:120741)