Fkbp5^{Gt(RRC236)Byg}

The pGT1Lxf gene trap vector that contains a splice acceptor and beta-geo was inserted into intron 4. Northern blot, quantitative RT-PCR and Western blot confirmed the expression of a fusion transcript and protein that lacked the peptidyl-prolyl cis/trans-isomerase-like domain and all three TPR domains critical for the protein's function. (J:120960)