设计了一个靶向载体,用于替换编码区的第2外显子和部分第3外显子,替换成抗氨苄青霉素的基因。 Western blot结果显示突变型睾丸中没有检测到蛋白(来源:J:121031)
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A targeting vector was designed to replace exon 2 and part of exon 3 with a neomycin resistance gene. Western blot confirmed absence of protein in mutant testis. (J:121031)