Tg(Crx-GFP,-ALPP)1Clc

A loxP-flanked kanamycin/neomycin drug resistance cassette and a GFP/IRES/PLAP fragment were targeted to replace the first exon of the mouse Crx gene in a BAC clone by ET-cloning. Cre was activated in the EL250 bacterial host strain with arabinose to remove the floxed kanamycin/neomycin segment, and the resultant BAC was digested with NotI to generate a transgenic fragment. (J:120988)