一个包含loxP位点和frt序列的筛选载体,带有诺如基因的neo cassette,被插入到了第五个内含子中。接着在3'非编码区也插入了一个loxP位点。随后通过Cre介导的neo cassette的敲除,删除了第六个外显子。基因敲除的确认通过基因组DNA的PCR检测完成。(来源:J:119939)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
基因内删除
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1
1
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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