4号和5号外显子被零/neo载体替换。通过 Western Blot 检测同源胚胎组织中无蛋白质表达,确认了零等位基因。(来源:J:120296)
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Exons 4 and 5 are replaced with a zeo/neo cassette. The null allele was confirmed by the absence of protein in a western blot of homozygous embryonic tissue. (J:120296)