在1号外显子的3'端,以反向方式插入了一种诺卡因抗性基因。编码在第一个甲硫氨酸下游124个bp处中断。突变脑组织的 Western blot 显示无蛋白质表达。(来源:J:119989)
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The 3' end of exon 1 was replaced with a neomycin resistance gene in the reverse orientation. Coding was interrupted 124 bp downstream of the first methionine. Western blot of mutant brain lysates confirmed absence of protein. (J:119989)