Del(16Dgcr2-Arvcf)1Ais

These mice were created by either breeding, or by targeting ES cells as follows. A targeting vector containing a PGK promoter, a loxP site and a neomycin resistance gene was inserted into the DiGeorge syndrome critical region gene 2, resulting in the loss of exons 4 and 5. These ES cells were additionally targeted with a vector containing two regions of the armadillo repeat gene deleted in velo-cardio-facial syndrome flanking a hygromycin resistance gene, a loxP site and a promoterless puromycin resistance gene. The insertion of this vector resulted in the loss of amino acids 83-276. Subsequent transient cre expression when the loxP sites were in cis resulted in the deletion of sequence between the two loxP sites. This 550-kb deletion encompasses 16 genes. (J:64380)