Single-cell embryos were coinjected with T-cell receptor alpha and T-cell receptor beta expression vectors containing the rearranged genomic Tcra and Tcrb VJ/V(D)J segments from the cytotoxic T-lymphocyte clone Y-1, which expresses a T cell receptor specific for the H2-Db-restricted SV40 large tumor antigen epitope I (amino acids 206-215). (J:84288)