一段包含2号外显子的1.2kb区域被替换为EGFP和一个含floxed neo cassette,通过 Western blot 对纯合小鼠胸腺细胞提取物的分析,证实了全长蛋白的缺失。(来源:J:116716)
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A 1.2 kb region that includes exon 2 was replaced with EGFP and a floxed neo cassette. Western blot analysis of homozygous thymus cell lysates confirmed absence of the full length protein. (J:116716)