在插入了抗氨苄青霉素抗性 cassette 后,对突变体的 Northern blot 检测证实了该编码 452-526 位氨基酸的 exons 未产生转录物。(来源:J:118426)
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A neomycin resistance cassette was inserted to disrupt the exon encoding amino acids 452-526. Northern blot confirmed absence of transcript in mutants. (J:118426)