去除1和2号外显子,包括起始翻译位点,通过Cre介导的重组。脾脏提取物的 Western blot 检测证实纯合子小鼠中无蛋白存在。(来源:J:117668)
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Exons 1 and 2 including the translation start site were removed by Cre mediated recombination. Western blot analysis of spleen lysates confirms absence of protein in homozygous mice. (J:117668)