大部分外显子1被反向插入了抗氨苄青霉素抗性基因 cassette。通过RT-PCR和 Western blot 验证了转录和蛋白的缺失。(来源:J:117799)
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Most of exon 1 was replaced with a neomycin resistance cassette in antisense orientation. Absence of transcript and protein were confirmed by RT-PCR and Western blot. (J:117799)