通过同源重组替换了一个包含ATG的PGK-neo cassette,随后通过Cre介导的重组手段移除。 Western blot在突变小鼠中未能检测到相应蛋白(来源:J:117646)
查看原文 参与反馈
A PGK-neo cassette replaced sequence containing the ATG by homologous recombination and was subsequently removed via cremediated recombination. Western blot failed to detect protein in mutant mice. (J:117646)