Krt14^{tm1(Krt10)Pko}

Sequence encoding the N-terminal head domain in exon 1 was replaced with the sequence for the keratin 10 head domain. Exons 7 and 8 were deleted and a cDNA encoding the keratin 10 tail domain was fused with exon 6 of keratin 14. A pgk-neo flanked by loxP sites was introduced into intron 1 and subsequently removed via cre mediated recombination. Expression of keratin 10 was detected by Western blotting, confirming successful creation of the chimeric protein. (J:117347)