设计了一个靶向载体,旨在替换包含ATG和整个锌指域的序列,用诺卡因抗性基因座替换。通过 Western blot 分析,证实了突变性垂体腺中蛋白质的缺失。(来源:J:116147)
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A targeting vector was desgned to replace sequence including the ATG and the entire zinc finger domain with a neomycin resistance cassette. Absence of protein in mutant pituitary glands was confirmed by Western blot analysis. (J:116147)