将GFP-lox-neo-lox cassette插入了所有已知剪接变体都共用的第2外显子中, Western blot结果显示突变体中没有蛋白质表达。(来源:J:116121)
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A GFP-lox-neo-lox cassette was inserted into exon 2, a common exon used by all known splice variants. Western blot confirmed absence of protein in mutants. (J:116121)