设计了一个靶向载体,目标去除编码第3-10个外显子(5-554个氨基酸)的序列,并在第4位氨基酸插入lacZ报告基因,使其在框架内表达。通过RT-PCR确认了转录本的缺失。免疫染色显示了lacZ基因的表达。(来源:J:115868)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S/SvEv
Targeted
插入,基因内删除
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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