在第六外显子中插入了5个碱基的缺失。 cassette中包含了一个floxed neo,随后通过Cre表达被去除。 Western blot确认了突变蛋白的存在。(来源:J:114752)
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A 5 bp deletion was engineered in exon 6. A floxed neo was included in the cassette and subsequently removed via cre expression. Western blot confirmed the presence of mutant protein. (J:114752)