在2-4号外显子被替换为一个带有floxed neo基因的序列,同时1号外显子被反转。之后,neo基因通过Cre介导的重组从该位点移除。 Western blot在突变性睾丸和小肠中未能检测到蛋白质。(来源:J:115256)
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Exons 2-4 were replaced with a floxed neo gene and exon 1 was reversed. The neo was subsequently removed from the locus via cre-mediated recombination. Western blot failed to detect protein in mutant testis and intestine. (J:115256)