使用了由tetracycline响应元件(tetO)控制的增强绿色荧光蛋白/Cre融合蛋白的tTA调控GFP::Cre构建,该构建中tetracycline调控的转录激活器(tTA)位于鼠Sp7基因(之前称为osterix或Osx1)的外显子1编码区域上游。通过转暂时表达flpe,从正确靶向的细菌中去除了带有frt标记的选择性 cassette。修改后的BAC(Osx1-GFP::Cre)的5.2kb片段用于对CD1胚胎的卵裂期细胞进行核移植。(来源:J:114494)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
CD-1
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290

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
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Year
IF
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